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Long-Chain Acyl-CoA Synthetase
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ATP + Coenzyme A |
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AMP + PPi |
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long-chain fatty acid |
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acyl-Coenzyme A |
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Locus Data & Links (May need to scroll right or left to show columns)
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LACS2
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Mutant Char.; Heterol Exp'n.
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4 Refs
Schnurr, J., Shockey, J., and Browse, J. (2004). The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis. Plant Cell 16 , 629-642.Bessire, M., Chassot, C., Jacquat, A.C., Humphry, M., Borel, S., Petetot, J.M.C., Metraux, J.P., and Nawrath, C. (2007). A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea. EMBO J. 26 , 2158-2168.Tang, D.Z., Simonich, M.T., and Innes, R.W. (2007). Mutations in LACS2, a long-chain acyl-coenzyme a synthetase, enhance susceptibility to avirulent Pseudomonas syringae but confer resistance to Botrytis cinerea in Arabidopsis. Plant Physiology 144 , 1093-1103.Pulsifer, I.P., Kluge, S., and Rowland, O. (2012). Arabidopsis LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1), LACS2, and LACS3 facilitate fatty acid uptake in yeast. Plant Physiology and Biochemistry 51 , 31-39.
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Protein family of 9 members; mutant have defected cuticle; cuticle is more permeable for dyes, interestingly leafs are more resistant to the pathogen Botrytis; LACS2 mutant has defected cuticle
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Data
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LACS3
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Seq. Similarity; Heterol Exp'n.
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3 Refs
Shockey, J.M., Fulda, M.S., Browse, J.A. (2002). Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism. Plant Physiol 129, 1710-22Lu, S., Song, T., Kosma, D.K., Parsons, E.P., Rowland, O., and Jenks, M.A. (2009). Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis. Plant J. 59 , 553-564.Pulsifer, I.P., Kluge, S., and Rowland, O. (2012). Arabidopsis LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1), LACS2, and LACS3 facilitate fatty acid uptake in yeast. Plant Physiology and Biochemistry 51 , 31-39.
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Data
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LACS9
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Plastid envelope: proteomics
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Heterol Exp'n.; Comple. of yeast mutant
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1 Ref
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Data
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LACS8
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Plastid envelope
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Heterol Exp'n.; Comple. of yeast mutant
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1 Ref
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Data
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LACS1
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Mutant Char.; Heterol Exp'n.; in vitro assays
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4 Refs
Shockey, J.M., Fulda, M.S., Browse, J.A. (2002). Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism. Plant Physiol 129, 1710-22Lu, S., Song, T., Kosma, D.K., Parsons, E.P., Rowland, O., and Jenks, M.A. (2009). Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis. Plant J. 59 , 553-564.Weng, H., Molina, I., Shockey, J., and Browse, J. (2010). Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis. Planta 231 , 1089-1100.Pulsifer, I.P., Kluge, S., and Rowland, O. (2012). Arabidopsis LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1), LACS2, and LACS3 facilitate fatty acid uptake in yeast. Plant Physiology and Biochemistry 51 , 31-39.
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Has overlapping function with lacs2; mutant shows defects both in wax and cutin monomer synthesis
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Data
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LACS6
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Peroxisome
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Mutant Char.
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2 Refs
Fulda, M., Schnurr, J., Abbadi, A., Heinz, E., and Browse, J. (2004). Peroxisomal acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana. Plant Cell 16 , 394-405.Shockey, J., and Browse, J. (2011). Genome-level and biochemical diversity of the acyl-activating enzyme superfamily in plants. Plant Journal 66 , 143-160.
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Essential for seedling development
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Data
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LACS5
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Heterol Exp'n.; Comple. of yeast mutant
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1 Ref
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Data
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LACS4
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Mutant Char.; Heterol Exp'n.; Comple. of yeast mutant
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2 Refs
Jessen, D., Olbrich, A., Knuefer, J., Krueger, A., Hoppert, M., Polle, A., and Fulda, M. (2011). Combined activity of LACS1 and LACS4 is required for proper pollen coat formation in Arabidopsis. Plant Journal 68 , 715-726.Shockey, J.M., Fulda, M.S., Browse, J.A. (2002). Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism. Plant Physiol 129, 1710-22
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lacs4 knockout has lower cuticular wax loads especially in combination with lacs1 mutants
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Data
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LACS7
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Peroxisome
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Mutant Char.
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2 Refs
Fulda, M., Schnurr, J., Abbadi, A., Heinz, E., and Browse, J. (2004). Peroxisomal acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana. Plant Cell 16 , 394-405.Shockey, J., and Browse, J. (2011). Genome-level and biochemical diversity of the acyl-activating enzyme superfamily in plants. Plant Journal 66 , 143-160.
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Essential for early seedling growth
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Data
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