Acyl Lipids: locations

ARABIDOPSIS
ACYL-LIPID METABOLISM

ENZYME & PROTEIN DATA

Triacylglycerol Lipase-Like (TAGL)

H₂O

free fatty acid

triacylglycerol (or diacylglycerol)

diacylglycerol (monoacylglycerol)

Brenda: [3.1.1.3]
IUBMB: [3.1.1.3]
ExPASy: [3.1.1.3]
ExplorEnz: [3.1.1.3]
PDB: [3.1.1.3]

Enzyme Pathways

TAG Degradation | Oxylipin I

Locus Data & Links (May need to scroll right or left to show columns)

Locus Id (TAIR Link)	Abbrev.	Mutant(s) (click for pop-up)	Pathway(s)	Subcelluar location	Evidence for function	Key References	Comments	ESTexpression in other oilseeds
At1g10740			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to Staphylococcus lipase BLAST score>100; Staphylococcus lipases exhibit both TAG lipase ; phospholipase A1 activities)	Data
At1g23330			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to Staphylococcus lipase BLAST score>100; Staphylococcus lipases exhibit both TAG lipase ; phospholipase A1 activities)	Data
At1g45201			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to castor bean acid lipase BLAST score>100)
At1g56630			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to castor bean acid lipase BLAST score>100)
At2g15230	LIP1	Atlip1	TAG Degradation		Mutant Char.; Heterol Exp'n.	2 Refs Karim, E.K., Stephanie, B., Emilia, O., Anne-Marie, G., Natalie, F., and Vincent, A. (2005). Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases. Febs Letters 579 ,6067-6073. El-Kouhen, K., Blangy, S., Ortiz, E., Gardies, A.-M., Ferte, N., and Arondel, V. (2005). Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases. FEBS Lett. 579 , 6067-6073.	Experimental: strong lipase activity of recombinant protein, no lipid phenotype of mutant	Data
At2g31690			TAG Degradation		Heterol Exp'n.	1 Ref Padham, A.K., Hopkins, M.T., Wang, T.W., McNamara, L.M., Lo, M., Richardson, L.G.L., Smith, M.D., Taylor, C.A., and Thompson, J.E. (2007). Characterization of a plastid triacylglycerol lipase from arabidopsis. Plant Physiol. 143 , 1372-1384.	Experimental: weak TAG lipase activity of recombinant protein. No lipid phenotype. Senescence associated phenotype. Protein colocalizes to plastoglobules
At3g14360			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to castor bean acid lipase BLAST score>100)	Data
At3g57140	SDP1-LIKE	sdp1-like	TAG Degradation		Mutant Char.	2 Refs Kelly AA, Quettier AL, Shaw E, Eastmond PJ. (2011). Seed storage oil mobilization is important but not essential for germination or seedling establishment in Arabidopsis. Plant Physiology 157 ,866-75. Quettier, A.-L., and Eastmond, P.J. (2009). Storage oil hydrolysis during early seedling growth. Plant Physiology and Biochemistry 47 , 485-490.	Previous enzyme ID : 164 (Patatin-like Acyl-Hydrolase) because of its Patatin domain; Experimental: lipid phenotype in double SDP1/SDP1-like mutant ,Strong homology to SDP1; enzyme activity not characterized; Double SDP1/SDP1L mutant seedlings cannot hydrolyze stored TAGs	Data
At5g04040	SDP1	sugar-dependent1 (sdp1)	TAG Degradation	Oilbody	Mutant Char.; Heterol Exp'n.	1 Ref Eastmond, P.J. (2006). SUGAR-DEPENDENT1 encodes a patatin domain triacylglycerol lipase that initiates storage oil breakdown in germinating Arabidopsis seeds. Plant Cell 18 , 665-675.	Previous enzyme ID : 164 (Patatin-like Acyl-Hydrolase) because of its Patatin domain; Experimental: strong lipase activity of recombinant protein, strong lipid phenotype of mutant, mutant impaired in postgerminative growth	Data
At5g14180	MPL1	mpl1	TAG Degradation		Mutant Char.; Seq. Similarity	1 Ref Louis, J., Lorenc-Kukula, K., Singh, V., Reese, J., Jander, G., and Shah, J. (2010). Antibiosis against the green peach aphid requires the Arabidopsis thaliana MYZUS PERSICAE-INDUCED LIPASE1 gene. Plant Journal 64 ,800-811.	Broad substrate specificity for TAG and phospholipids. Seq. similarity (to AtLIP1 ; human gastric ; lysosomal lipases BLAST score>100).	Data
At5g14930	SAG101	sag101	TAG Degradation		Mutant Char.; Heterol Exp'n.	2 Refs He, Y.H., and Gan, S.S. (2002). A gene encoding an acyl hydrolase is involved in leaf senescence in Arabidopsis. Plant Cell 14 , 805-815. Feys, B.J., Wiermer, M., Bhat, R.A., Moisan, L.J., Medina-Escobar, N., Neu, C., Cabral, A., and Parker, J.E. (2005). Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity. Plant Cell 17 , 2601-2613.	Experimental: weak TAG lipase activity of recombinant protein. No lipid phenotype. Senescence associated phenotype	Data
At5g18630			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to Rhizopus lipase BLAST score=100)	Data
At5g18640			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to Rhizopus lipase BLAST score=100)	Data
At5g42930			TAG Degradation		Seq. Similarity		Seq. similarity ONLY (to castor bean acid lipase BLAST score>100)	Data
At5g67050			TAG Degradation Oxylipin I Oxylipin II		Seq. Similarity		Seq. similarity ONLY (to castor bean acid lipase BLAST score>100)	Data

Triacylglycerol Lipase-Like (TAGL)

Enzyme Pathways

Locus Data & Links (May need to scroll right or left to show columns)