Acyl Lipids: locations

ARABIDOPSIS
ACYL-LIPID METABOLISM

ENZYME & PROTEIN DATA

Midchain Alkane Hydroxylase

NADH+ H⁺+ O₂

H₂O +NAD⁺

nonacosane

Reaction

nonacosan-15-ol

IUBMB: [1.14.13.*] [1.1.1.-]

Enzyme Pathways

FA Elong & Wax

Locus Data & Links (May need to scroll right or left to show columns)

Locus Id (TAIR Link)	Abbrev.	Mutant(s) (click for pop-up)	Pathway(s)	Subcelluar location	Evidence for function	Key References	Comments	Microarray Expression	Proteomics data	ESTexpression in other oilseeds
At1g47620	CYP96A8		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At1g47630	CYP96A7		FA Elong & Wax				Pseudogene
At1g57750	CYP96A15 / MAH1	mah1, cyp96a15	FA Elong & Wax	Endomembrane (GFP)	Mutant Char.; in-planta Overexp'n.	2 Refs Greer, S., Wen, M., Bird, D., Wu, X.M., Samuels, L., Kunst, L., and Jetter, R. (2007). The cytochrome p450 enzyme CYP96A15 is the midchain alkane hydroxylase responsible for formation of secondary alcohols and ketones in stem cuticular wax of arabidopsis. Plant Physiol. 145 , 653-667. Wen, M., and Jetter, R. (2009). Composition of secondary alcohols, ketones, alkanediols, and ketols in Arabidopsis thaliana cuticular waxes. J. Exp. Bot. 60 , 1811-1821.	Likely a midchain alkane hydroxylase (MAH1). Proposed to act indirectly as the ketone-forming enzyme by a second hydroxylation on the same carbon, forming an unstable geminal diol, spontaneously forming the ketone. This latter hypothesis remains to be demonstrated.
At1g65340	CYP96A3		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At2g21910	CYP96A5		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At2g23180	CYP96A1		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At4g32170	CYP96A2		FA Elong & Wax		Seq. Similarity	1 Ref Lu, C., Xin, Z., Ren, Z., Miquel, M., and Browse, J. (2009). An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis. Proc. Natl. Acad. Sci. USA 106 , 18837-18842.	Seq. Similarity to MAH1
At4g39480	CYP96A9		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At4g39490	CYP96A10		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At4g39500	CYP96A11		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At4g39510	CYP96A12		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At5g02900	CYP96A13		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1
At5g52320	CYP96A4		FA Elong & Wax		Seq. Similarity		Seq. Similarity to MAH1