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Phosphatidate Phosphatase
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H2O |
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Pi |
1,2-diacylglycerol-phosphate |
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1,2-diacylglycerol |
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Locus Data & Links (May need to scroll right or left to show columns)
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LPP2
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Mutant Char.; Heterol Exp'n.
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2 Refs
Pierrugues, O., Brutesco, C., Oshiro, J., Gouy, M., Deveaux, Y., Carman, G.M., Thuriaux, P., and Kazmaier, M. (2001). Lipid phosphate phosphatases in Arabidopsis?Regulation of the AtLPP1 gene in response to stress. J. Biol. Chem. 276 , 20300-20308.Katagiri, T., Ishiyama, K., Kato, T., Tabata, S., Kobayashi, M., and Shinozaki, K. (2005). An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43 , 107-117.
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Heterologous protein exhibits both PA and DGPP phosphatase activity(Ref1), mutant accumulates PA during germination and is hypersensitive to ABA (Ref2)
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Data
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LPP1
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Plastid
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Mutant Char.; Heterol Exp'n.
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2 Refs
Pierrugues, O., Brutesco, C., Oshiro, J., Gouy, M., Deveaux, Y., Carman, G.M., Thuriaux, P., and Kazmaier, M. (2001). Lipid phosphate phosphatases in Arabidopsis?Regulation of the AtLPP1 gene in response to stress. J. Biol. Chem. 276 , 20300-20308.Franca, M.G.C., Matos, A.R., D'Arcy-Lameta, A., Passaquet, C., Lichtle, C., Zuily-Fodil, Y., and Pham-Thi, A.T. (2008). Cloning and characterization of drought-stimulated phosphatidic acid phosphatase genes from Vigna unguiculata. Plant Physiology and Biochemistry 46 , 1093-1100.
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Heterologous protein exhibits both PA and DGPP phosphatase activity
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Data
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PP; AtLPP3
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Mitochondria
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Seq. Similarity
Seq. Similarity; Heterol Exp'n.
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3 Refs
Pierrugues, O., Brutesco, C., Oshiro, J., Gouy, M., Deveaux, Y., Carman, G.M., Thuriaux, P., and Kazmaier, M. (2001). Lipid phosphate phosphatases in Arabidopsis?Regulation of the AtLPP1 gene in response to stress. J. Biol. Chem. 276 , 20300-20308.Katagiri, T., Ishiyama, K., Kato, T., Tabata, S., Kobayashi, M., and Shinozaki, K. (2005). An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43 , 107-117.Paradis, S., Laura Villasuso, A., Aguayo, S.S., Maldiney, R., Habricot, Y., Zalejski, C., Machado, E., Sotta, B., Miginiac, E., and Jeannette, E. (2011). Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves. Plant Physiology and Biochemistry 49 , 357-362.
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Data
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PAH1
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Endomembrane
Cytosol
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Mutant Char.; Heterol Exp'n.; Seq. Similarity; Comple. of yeast mutant
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4 Refs
Han, G.S., Wu, W.I., and Carman, G.M. (2006). The Saccharomyces cerevisiae lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme. J. Biol. Chem. 281 , 9210-9218.Nakamura, Y., Koizumi, R., Shui, G., Shimojima, M., Wenk, M.R., Ito, T., and Ohta, H. (2009). Arabidopsis lipins mediate eukaryotic pathway of lipid metabolism and cope critically with phosphate starvation. Proc. Natl. Acad. Sci. USA 106 , 20978-20983.Eastmond, P.J., Quettier, A.L., Kroon, J.T.M., Craddock, C., Adams, N., and Slabas, A.R. (2010). Phosphatidic acid phosphohydrolase 1 and 2 regulate phospholipid synthesis at the endoplasmic reticulum in Arabidopsis. Plant Cell 22 ,2796-2811.Mietkiewska, E., Siloto, R.M.P., Dewald, J., Shah, S., Brindley, D.N., and Weselake, R.J. (2011). Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1 Delta mutant strain of Saccharomyces cerevisiae. Febs Journal 278 , 764-775.
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Seq. Similarity to yeast PAH1; Complements yeast PAP mutant; mutant characterization;pah1 pah2 double mutant has decreased soluble PAP activity and during phosphate starvation phospholipid remodelling to DGDG i decreased
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Data
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LPP4
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Seq. Similarity
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1 Ref
Katagiri, T., Ishiyama, K., Kato, T., Tabata, S., Kobayashi, M., and Shinozaki, K. (2005). An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43 , 107-117.
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Data
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LPP ε1
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Plastid
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Mutant Char.; Heterol Exp'n.
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1 Ref
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Plastidial localization, double mutant lpp-1lpp-2 no change in bulk glycerolipid metabolism
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Data
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LPP δ
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Endomembrane (GFP)
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Seq. Similarity
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3 Refs
Marion, J., Bach, L., Bellec, Y., Meyer, C., Gissot, L., and Faure, J.D. (2008). Systematic analysis of protein subcellular localization and interaction using high-throughput transient transformation of Arabidopsis seedlings. Plant Journal 56 , 169-179.Tsegaye, Y., Richardson, C.G., Bravo, J.E., Mulcahy, B.J., Lynch, D.V., Markham, J.E., Jaworski, J.G., Chen, M., Cahoon, E.B., and Dunn, T.M. (2007). Arabidopsis mutants lacking long chain base phosphate lyase are fumonisin-sensitive and accumulate trihydroxy-18:1 long chain base phosphate. J. Biol. Chem. 282 , 28195-28206.Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.
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suggested function:LCB-P phosphatases (Ref2), non-plastidial localization (Ref 3)
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Data
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LPP β
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Seq. Similarity
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1 Ref
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Data
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LPP γ
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Plastid
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Mutant Char.; Heterol Exp'n.; Seq. Similarity
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2 Refs
Tsegaye, Y., Richardson, C.G., Bravo, J.E., Mulcahy, B.J., Lynch, D.V., Markham, J.E., Jaworski, J.G., Chen, M., Cahoon, E.B., and Dunn, T.M. (2007). Arabidopsis mutants lacking long chain base phosphate lyase are fumonisin-sensitive and accumulate trihydroxy-18:1 long chain base phosphate. J. Biol. Chem. 282 , 28195-28206.Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.
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Plastidial localization, single mutant lpp- lethal (Ref1), suggested function:LCB-P phosphatases (Ref2). ,Phosphatidate phosphatase activity reported Nakamura et al (2007).Plastidial localization, double mutant lpp-1lpp-2 no change in bulk glycerolipid metabolism.
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Data
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PAH2
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Cytosol
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Mutant Char.; Heterol Exp'n.; Seq. Similarity; Comple. of yeast mutant
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4 Refs
Han, G.S., Wu, W.I., and Carman, G.M. (2006). The Saccharomyces cerevisiae lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme. J. Biol. Chem. 281 , 9210-9218.Nakamura, Y., Koizumi, R., Shui, G., Shimojima, M., Wenk, M.R., Ito, T., and Ohta, H. (2009). Arabidopsis lipins mediate eukaryotic pathway of lipid metabolism and cope critically with phosphate starvation. Proc. Natl. Acad. Sci. USA 106 , 20978-20983.Eastmond, P.J., Quettier, A.L., Kroon, J.T.M., Craddock, C., Adams, N., and Slabas, A.R. (2010). Phosphatidic acid phosphohydrolase 1 and 2 regulate phospholipid synthesis at the endoplasmic reticulum in Arabidopsis. Plant Cell 22 ,2796-2811.Mietkiewska, E., Siloto, R.M.P., Dewald, J., Shah, S., Brindley, D.N., and Weselake, R.J. (2011). Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1 Delta mutant strain of Saccharomyces cerevisiae. Febs Journal 278 , 764-775.
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Seq. Similarity to yeast PAH1; Complements yeast PAP mutant; mutant characterization;pah1 pah2 double mutant has decreased soluble PAP activity and during phosphate starvation phospholipid remodelling to DGDG i decreased
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Data
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LPP ε2
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Plastid
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Mutant Char.; Heterol Exp'n.
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1 Ref
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Plastidial localization, double mutant lpp-1lpp-2 no change in bulk glycerolipid metabolism
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Data
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