Acyl Lipids: enzymes

ARABIDOPSIS
ACYL-LIPID METABOLISM

ENZYME & PROTEIN DATA

Acyl-CoA Desaturase-like

monogalactosyldiacylglycerol (palmitate-containing)

Reaction_

monogalactosyldiacylglycerol (16:1 δ7-containing)

IUBMB: [1.14.99.*]

Enzyme Pathways

Euk Gal/Sulpho

Locus Data & Links (May need to scroll right or left to show columns)

Locus Id (TAIR Link)	Abbrev.	Mutant(s) (click for pop-up)	Pathway(s)	Subcelluar location	Evidence for function	Key References	Comments	Microarray Expression	Proteomics data	ESTexpression in other oilseeds
At1g06080		ads1				2 Refs Smith, M.A., Dauk, M., Ramadan, H., Yang, H., Seamons, L.E., Haslam, R.P., Beaudoin, F., Ramirez-Erosa, I., and Forseille, L. (2013). Involvement of Arabidopsis ACYL-COENZYME A DESATURASE-LIKE2 (At2g31360) in the Biosynthesis of the Very-Long-Chain Monounsaturated Fatty Acid Components of Membrane Lipids. Plant Physiology 161 ,81-96. Heilmann, I., Pidkowich, M.S., Girke, T., and Shanklin, J. (2004a). Switching desaturase enzyme specificity by alternate subcellular targeting. Proc. Natl. Acad. Sci. USA 101 , 10266-10271.	Endogneous substrates unknown; Plants deficient in AtADS1 expression had reduced levels of 26:1( n -9) in seed lipids, but no significant changes in leaf phospholipids or sphingolipids			Data
At1g06090						1 Ref Heilmann, I., Mekhedov, S., King, B., and Shanklin, J. (2004). Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype. Plant Physiol 136 , 4237-4245.
At1g06100						1 Ref Heilmann, I., Mekhedov, S., King, B., and Shanklin, J. (2004). Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype. Plant Physiol 136 , 4237-4245.
At1g06120						1 Ref Heilmann, I., Mekhedov, S., King, B., and Shanklin, J. (2004). Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype. Plant Physiol 136 , 4237-4245.
At1g06350						1 Ref Heilmann, I., Mekhedov, S., King, B., and Shanklin, J. (2004). Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype. Plant Physiol 136 , 4237-4245.
At1g06360						1 Ref Heilmann, I., Mekhedov, S., King, B., and Shanklin, J. (2004). Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype. Plant Physiol 136 , 4237-4245.				Data
At2g31360		ads2	Euk Gal/Sulpho	Endomembrane	Mutant Char.; Heterol Exp'n.; Seq. Similarity	2 Refs Chen, M.J., and Thelen, J.J. (2013). ACYL-LIPID DESATURASE2 Is Required for Chilling and Freezing Tolerance in Arabidopsis. Plant Cell 25 ,1430-1444. Heilmann, I., Pidkowich, M.S., Girke, T., and Shanklin, J. (2004a). Switching desaturase enzyme specificity by alternate subcellular targeting. Proc. Natl. Acad. Sci. USA 101 , 10266-10271.	ads2 mutant plants appear similar to the wild type under standard growth conditions but display a dwarf and sterile phenotype when grown at 6°C. Fatty acid composition analysis demonstrated that ads2 mutant plants at 6°C have reduced levels of 16:1, 16:2, 16:3, and 18:3 and higher levels of 16:0 and 18:0 fatty acids compared with the wild type. In vitro expression in yeast shows desaturase activity (n-9 position) on very long chain fatty acid (VLCFA) substrates			Data
At3g15870					Seq. Similarity	1 Ref Heilmann, I., Mekhedov, S., King, B., and Shanklin, J. (2004). Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype. Plant Physiol 136 , 4237-4245.				Data